Frequently Asked Questions
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FAQ’s
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Yes! Please see Tech Report 202: Viewing Cells on Flexcell®'s Culture Plates. Light and Electron Microscope Techniques and Immunofluorescent Imaging. NOTE: Mounting medium needs to be water based.
If your lab is in Canada or the United States, we do offer 6-month and 12-month renewable leases. Please contact our Flexcell® Sales Team at info@Flexcellint.com for more details.
If your lab is in Canada or the United States, we offer 6-week complimentary trial period for all equipment. Contact the Flexcell® Sales team to learn more details.
Yes, Flexcell® products are sold worldwide. Flexcell® International takes all order for Australia, Canada, United States, South America and Israel. We have International regional distributors. Please contact Flexcell® at info@Flexcellint.com or send an online request via the website for distributors in your global region.
Yes! Flexcell® periodically has sales on equipment and consumables. Please contact Flexcell®'s Sales Department for current promotions.
As of 2024, we have over 7,000 science publications worldwide using our products. You can search our Publication database on our Publications page by year, by equipment used, organ, cell and tissue type and research focus. Another search resource is Pubmed or Google Scholar using the Flexcell® product name and cell or tissue type.
The grease can be removed with the following protocol after the cells have been fixed:
- Using a dry cotton swab, wipe off as much of the excess lubricant as possible. This may require a couple of swabs. Only a thin film of lubricant should remain on the membrane surface.
- Wet the tip of a new cotton swab with a mild cleaning solution such as Formula 409, window cleaner, or hand soap. Rub the wet swab gently over the entire surface of the well. Repeat with a new cotton swab and fresh cleaning solution as necessary to remove all residual lubricant from the membrane surface.
- Rinse the cleaned surface with deionized water using a spray, wash, or dispensing bottle.
- Blot the surface dry with a Kimwipe or other lint-free wipe.
Also, please see Tech Report 202 for more techniques on viewing cells on Flexcell® culture plates.
Please see Tech Report 106: Matrix Bonded Growth Surfaces: Growing Cells in a More Natural Matrix Environment, which discusses the various matrix coatings available. All Flexcell® culture plates are sterilized and individually packaged for ease of use.
Strain is applied to cells via vacuum deformation of the silicone elastomer growth substrate in the rubber-bottomed culture plates as depicted in the schematic below (Figure 1). Please also see our applications page about Applying Mechanical Load to Cells in Monolayer Culture and Tech Report 101: Loading Stations™. Quantification of Strain on the Membrane Surface for further information.
The plates you received have a different calibration file from previous plates. If your system has not been updated with the new calibration files, they you will need to contact Flexcell® to get the software update for your system. Please be sure to include your unit serial number when contacting Flexcell®.
Flexcell® can take your Request for quote online. We accept major credit cards, ACH bank transfers or checks.
BioFlex® 6-well, HT BioFlex® 24-well, CellSoft® BioFlex® 6-well and UniFlex® culture plates are used for 2D Monolayer cell culture.
BioFlex®, HT BioFlex®, CellSoft® BioFlex® and Tissue Train® circular foam culture plates are used to apply equibiaxial load.
UniFlex® and Tissue Train® Linear (Cerex or foam anchors) are used to apply uniaxial load.
All our Tissue Train® culture plates are used to create 3D constructs in either circular/spheroid or linear shapes. If you are interested in collaborating on a custom 3D shape, please contact our Flexcell® Sales Team directly at info@Flexcellint.com
BioFlex®, HT BioFlex®, and Circular Foam Tissue Train® plates can be used during application of equibiaxial tension. Linear and Trapezoidal Tissue Train® plates as well as UniFlex® plates are used during application of uniaxial tension.
BioFlex®, HT BioFlex®, and UniFlex® plates are used to culture cells in monolayer. Tissue Train® culture plates are used for creating 3D bioartifical cell-seeded constructs.
This may be caused by plating too many cells. Plate with a lower cell density. If the cells are not confluent, they will form a matrix with the collagen (or other matrix coating) attaching more securely to the growth surface.
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